17+ a modular cloning toolkit for genome editing in plants
NTC is the no template control - A modular cloning toolkit for genome. The three panels shown come from different parts of the same DNA gel Additional file 4.
A Modular Cloning Toolkit For Genome Editing In Plants Biorxiv
A Modular Cloning Toolkit for Genome Editing in Plants Florian Hahn1 Andrey Korolev12 Laura Sanjurjo Loures1 and Vladimir Nekrasov1 The Dawn of the Crispr-Free Genome Editing Era.
. Results Here we present an expanded cloning toolkit that contains 103 modules encoding a variety of CRISPRCas-based nucleases and their corresponding guide RNA backbones. Here Hahn et al. PDF Background CRISPRCas has recently become a widely used genome editing tool in various organisms including plants.
We believe the presented CRISPRCas toolkit is a great resource that will contribute towards wider adoption of the CRISPRCas genome editing technology and. The modular cloning kit presented in the study enables quick and facile assembly of DNA constructs for genome editing applications in plants and is an addition to previously published collections of compatible GG modules 7 911. M is the DNA marker.
The kit includes modules encoding a number of CRISPRCas nucleases SaCas9 StCas9 LbCas12a etc that could be used as an. Have developed an additional cloning toolkit which contains ninety-nine modules encoding several CRISPRCas nucleases and their cognate guide RNA. Find read and cite.
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